The liver microsomal hydroxylation enzyme system. Induction and properties of the functional components.
نویسندگان
چکیده
Enzymology of the Liver Frontiers of Gastrointestinal Research Vol. 2 Series Editor: LEO VAN DER RBIS, San Francisco, Calif. Editorial Board: R. LAMBERT, Lyon; S. LORBER, Philadelphia, Pa.; D. W. PIPER, Crows Nest, N.S.W.; H. SHIRAKABE, Tokyo; K.B. TAYLOR, Palo Alto, Calif.; N. ZLLNER, Munich S. Karger Basel' Mnchen . Paris' London' New York' Sydney Enzymology of the Liver Editor: LEO VAN DER REIS, San Francisco, Calif. 20 figures and 39 tables, 1976 S. Karger Basel Mnchen Paris London New York Sydney Frontiers of Gastrointestinal Research Vol. 1: Immune Disorders. Ed. LEO VAN DER REIS (San Francisco, Calif.) IX + 187 p., 21 fig., 8 tab., 1975 ISBN 3-8055-1751-3 Cataloging in Publication Enzymology of the liver Editor: Leo van der Reis. Basel, New York, Karger (cI976) (Frontiers of gastrointestinal research, v.2) 1. Liver enzymology I. Van der Reis, Leo, ed. II. Title III. Series W1 FR946E v.2/WI 700 E61 ISBN 3-8055-2220--7
منابع مشابه
Properties of partially purified liver microsomal cytochrome P-450: acceptance of two electrons during anaerobic titration.
A large variety of foreign compounds, including drugs, petroleum products and insecticides, as well as physiological substrates such as fatty acids and steroids, are attacked by molecular oxygen in the presence of NADPH, a reductase and liver microsomal cytochrome P-450 (P-450LM) as the oxygenating catalyst. Although much has been learned about the hydroxylation reactions catalyzed by liver mic...
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As described elsewhere (1-3), studies in this laboratory have led to the solubilization of liver microsomal cytochrome P-450 by treatment with deoxycholate and to its separation by ion exchange chromatography from NA DPH-cytochrome P450 reductase and a heat-stable lipid fraction. All three components are required, as well as mcilecular oxygen and NADPH, for the hydroxylation of drugs (4, 5), fa...
متن کاملCharacterization of partially purified cytochromes P-450 and P-448 from rat liver microsomes.
The liver microsomal hydroxylation enzyme system has been resolved into three components: cytochrome P-450, an NADPH-dependent reductase, and a lipid identified as phosphatidylcholine (1, 2). Using the reconstituted hydroxylation system, we have recently shown that the enzyme systems prepared from PB-’ or 3-MCtreated immature, male rats exhibit different substrate specificities and that such sp...
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Rat liver microsomal testosterone (250 microM) hydroxylation and immunoreactive CYP3A protein were compared after administration of the antiglucocorticoid RU 486 (50 mg.kg-1.day-1 for 4 days) and the hypocholesterolaemic drug SR-12813 (150 mg.kg-1.day-1 for 4 days). Markers of CYP3A-mediated enzyme activity (testosterone 15 beta-, 6 beta-, and 2 beta-hydroxylation) were increased after administ...
متن کاملHydroxylation of benzphetamine and other drugs by a solubilized form of cytochrome P-450 from liver microsomes: lipid requirement for drug demethylation.
A solubilized hepatic microsomal enzyme system previously shown to catalyze the w-hydroxylation of fatty acids also catalyzes the hydroxylation of drugs. Benzphetamine, aminopyrine, ethylmorphine, hexobarbital, norcodeine, and p-nitroanisole undergo aerobic demethylation in the presence of NADPH and the resolved enzyme system. The required submicrosomal components for benzphetamine demethylatio...
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ورودعنوان ژورنال:
- Frontiers of gastrointestinal research
دوره 2 شماره
صفحات -
تاریخ انتشار 1976